Regulatory

Part:BBa_K1820010

Designed by: Alex Torgesen   Group: iGEM15_Utah_State   (2015-09-16)

CP44

Synthetic promoter created for use in Lactococcus lactis but also shows activity in E. coli, part BBa_K1033225 from the 2013 Uppsala team. Of the three promoters that we chose to use; cp8, cp11, and cp44; this one had the lowest activity. It does not contain a ribosomal binding site. The paper listed below has more information.

Jensen, Peter Ruhdal and Karin Hammer. "The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters." Applied and Environmental Microbiology (1998): 82-87. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/]

To visit the original page for this part, visit https://parts.igem.org/Part:BBa_K1033225

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None